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Microspores of heading Chinese cabbage, Korean turnip and their F, hybrids were cultured in several revised media of the modified NLN medium. More embryos were induced in Chinese cabbage when cultured in the hormone free NLN10 medium supplemented with 10% sucrose compared to the NLN10PGR medium supplemented with NAA 0.5 ㎎/L and BA 0.05 ㎎/L. But, in the case of Korean turnip and F₁ hybrid, the NLN10PGR was much better than the hormone free NLN10. Only 35 out of 421 embryos had been transplanted to the plant regeneration medium, MS2 (MS basic medium + 2% sucrose), developed into the normal plant. However, over 95% of abnormal plant regenerated from embryos recovered normal morphology when they were subcultured on the MSK medium (MS basic medium + NAA 0.05 ㎎/L + BA 1.0 ㎎/L + sucrose 3%). A total of 209 plants were divided into 30 haploids, 147 dihaploids, 16 tetrads, and 16 others by the flower size, pollen abundance and self fertility.
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